Fig. 4.

Electroporation is an effective method for siRNA uptake in bovine monocyte-derived macrophages (bMDM) without inducing a type I interferon (IFN) response. bMDM were electroporated without siRNA (EC), with MEFV siRNA or non-target control siRNA (NTC siRNA). (A) After 48 h the electroporated bMDM were activated with 100 ng/ml LPS for 2 h. The results are expressed as the percentage MEFV mRNA detected compared to that measured in untreated cells (NC). Error bars denote the variation between three technical replicates. (B) After 48 h cells were harvested and mRNA levels of the IFN-response gene interferon-induced protein with tetratricopeptide repeats 1 (IFIT1) were measured by qRT-PCR. The results are expressed as the IFIT1 mRNA fold difference compared to that measured in NC. Error bars denote the variation between three technical replicates. The results are representative of three repeat experiments using bMDM from different animals.