Total RNA (A) and protein (B) extracted at indicated developmental time points from dfmr1 null (dfmr150M) and control (w1118). Bars show the mean μg per head ± standard deviation. Significance: 0.05>p>0.01 (*), 0.01<p<0.001 (**). Stages: P3 (60–70 hrs APF), P4 (88–96 hrs APF), 0–7 hrs AE, 1d (21–24 hrs AE), 4d (96–112 hrs AE), 9d (216–232 hrs AE).