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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Neurobiol Dis. 2014 Jan 12;65:142–159. doi: 10.1016/j.nbd.2014.01.008

Figure 2. Brain GABAergic neurons express dFMRP.

Figure 2

A) Brain immunocytochemistry to visualize GAD-Gal4-driven UAS-mCD8::GFP (green) at the membrane and cytoplasmic anti-dFMRP labeling (red) from anterior (A) and posterior (P) perspectives (y1w*/+; GAD-Gal4/UAS-mCD8::GFP.L.LL5). Optic and antennal lobes (OL and AL, respectively) and MB calyx (C, arrowhead) are indicated for reference. Higher magnification views of single optical slices show antennal lobe (A, boxed) and calyx regions (P, boxed). B) Brain immunocytochemistry to visualize GAD-Gal4-driven nuclear UAS-GFP.nls (NLS-GFP, green) and anti-dFMRP labeling (red) from both anterior (A) and posterior (P) (w1118/+; GAD-Gal4/UAS-GFP.nls.14). The dashed box denotes the MB calyx region shown at higher magnification in the second row. GABAergic neurons in all brain areas express dFMRP. Arrowheads denote neurons displaying GAD-Gal4-driven UAS-GFP.nls with associated dFMRP. Arrows denote dFMRP-expressing neurons not within the GAD-Gal4 population.