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. Author manuscript; available in PMC: 2014 Apr 16.
Published in final edited form as: J Am Chem Soc. 2011 Nov 18;133(49):19878–19888. doi: 10.1021/ja207907d

Figure 2.

Figure 2

PCR fidelity determination via gel mobility. Based on the intensity of the DNA band that shifts in the presence of added streptavidin (SA), the biotin incorporation level is 65% for dMMO2SSBIO-d5SICS, 64% for dNaM-d5SICSSSBIO, and 72% for dNaM-d5SICSBIO. (Note that the slightly slower migrating band in lane 1 corresponds to unbiotinylated single-stranded DNA resulting from incomplete annealing after PCR.) The overall incorporation levels are converted to fidelities (Table 2) by normalizing by the number of doublings. A 50 bp DNA ladder is loaded in the rightmost lane.