. Author manuscript; available in PMC: 2014 Apr 16.

Published in final edited form as: J Am Chem Soc. 2011 Nov 18;133(49):19878–19888. doi: 10.1021/ja207907d

Table 2.

PCR fidelities and amplification efficiencies.^a

dXTPs incorporated	Amplification	Fidelity (sequencing)^b	Fidelity (gel-shift)^c
dNaM and d5SICS	735	>99.7	-
dMMO2 and d5SICS	609	99.6	-
dNaM and d5SICS_αS^d	662	99.6	-
dMMO2^PA and d5SICS	489	>99.7	-
dMMO2^A, d5SICS	528	99.5	-
dNaM and d5SICS^PA	888	>99.7	-
dNaM and d5SICS^A	160	91.1	-
dMMO2^PA and d5SICS^PA	960	>99.7	-
dMMO2^A and d5SICS^A	279	90.9	-
dMMO2^A and d5SICS^PA	624	98.7	-
dMMO2^PA and d5SICS^A	167	91.4	-
dMMO2^A and d5SICS_α_S^d	378	99.4	-
dMMO2^SSBIO and d5SICS	351	99.2	95.0
dNaM and d5SICS^SSBIO	690	>99.7	95.4
dNaM and d5SICS^BIO	624	>99.7	96.5
dNaM only	164^e	-^e	-
d5SICS only	217^e	-^e	-

See Materials and Methods for experimental details.

Calculated as average fidelities in both directions per doubling (see Materials and Methods for details).

Calculated from gel mobility assay (see text).

d5SICS_αS was used as a mixture of Sp and Rp diastereomers.

Unnatural base pair lost during amplification.