Table 3.

Nucleotide composition analysis of T7 RNAP transcription products.^a

		Normalized composition of nucleotides incorporated 5’ to A during transcription of 17 nt RNA.
Template	XTP	Ap	Gp	Cp	Up	Yp
d5SICS	dMMO2A	1.02 ± 0.01 [1]	1.94 ± 0.03 [2]	n.d. [0]b	n.d. [0]b	1.03 ± 0.02 [1]
d5SICS	dMMO2PA	0.99 ± 0.03 [1]	1.99 ± 0.03 [2]	n.d. [0]b	n.d. [0]b	1.01 ± 0.04 [1]
dNaM	d5SICSA	1.03 ± 0.02 [1]	2.00 ± 0.01 [2]	n.d. [0]b	n.d. [0]b	0.96 ± 0.02 [1]
dNaM	d5SICSPA	1.07 ± 0.01 [1]	1.95 ± 0.02 [2]	n.d. [0]b	n.d. [0]b	0.95 ± 0.01 [1]
		Normalized composition of nucleotides incorporated 5’ to A during transcription of ${\mathrm{tRNA}}_{\mathrm{CUA}}^{\mathrm{Tyr}}$.
Template	XTP	Ap	Gp	Cp	Up	Yp
d5SICS	MMO2A	4.06 ± 0.13 [4]	3.03 ± 0.07 [3]	5.91 ± 0.17 [6]	0.98 ± 0.03 [1]	1.02 ± 0.05 [1]
dNaM	5SICSA	4.16 ± 0.16 [4]	3.10 ± 0.04 [3]	5.86 ± 0.06 [6]	1.01+0.02 [1]	0.92 ± 0.04 [1]

^aValues were determined by dividing the radioactivity observed for a particular monophosphate by the product of the total radioactivity and the expected number of nucleotides. Predicted values assuming 100% fidelity are show in brackets. The error reported is the standard deviation of at least three independent determinations.

^bBelow detection limit.