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. 2014 Jan 4;57(2):449–461. doi: 10.1021/jm401508p

Figure 3.

Figure 3

Mode of inhibition of CB7 toward ALDH3A1. (A) Lineweaver–Burk representation of the nonlinear fit to the competitive inhibition equation for CB7 (0–125 nM) versus varied benzaldehyde (100–1000 μM) at fixed coenzyme concentration NADP+ (1.5 mM). (B) Lineweaver–Burk representation of the nonlinear fit to the noncompetitive inhibition equation for CB7 (0–200 nM) toward varied NADP+ (100–500 μM) at fixed benzaldehyde (1 mM). All experiments were performed in triplicate, and one representative experiment out of three is depicted.