Table 3. Kinetics Data for ALDH3A1 and the Q122A and Q122W Mutant Enzymes.
KMbenzaldehyde (μM) | kcat/KM (min–1 μM–1) | KiCB7 (μM) | |
---|---|---|---|
WT | 279 ± 23 | 4.91 ± 0.25 | 0.2 |
Q122A | 425 ± 38 | 3.2 ± 0.13 | 0.2 |
Q122W | 257 ± 35 | 1.73 ± 0.27 | NI |
KMbenzaldehyde (μM) | kcat/KM (min–1 μM–1) | KiCB7 (μM) | |
---|---|---|---|
WT | 279 ± 23 | 4.91 ± 0.25 | 0.2 |
Q122A | 425 ± 38 | 3.2 ± 0.13 | 0.2 |
Q122W | 257 ± 35 | 1.73 ± 0.27 | NI |