Figure 2.

Surface-Contoured Structure of Complex between scFv13R4 and β-gal

(A) Surface-contoured 3D map of β-gal alone (blue) and the difference map between β-gal and the β-gal:Fv complex (pink) with docked coordinates 1F4A of β-gal (Juers et al., 2000) and a typical Fv structure, taken from the 3A6B complex (Yokota et al., 2010) between hen egg lysozyme and a similar Fv. The maps were calculated using Frealign. The antibody domains are contoured at two different contour levels, showing the whole Fv domain at a lower contour level (pink) and the (just) resolved heavy and light chain densities at a higher contour level (green).

(B) FSC plots for the β-gal:Fv complex. In blue squares, the FSC between experimental maps each calculated from half of the single-particle images shows a resolution at FSC 0.143 of ∼13 Å. In red circles, FSC between the 3D map and the density from the molecular model shows a resolution at FSC 0.5 of ∼15 Å. Figure S2 shows a similar 3D map and FSC plot for the structure computed using Relion.