Fig. 1.

Dissociated hippocampal cells after 3 days in culture contain both βIII tubulin-positive neurons and GFAP-positive astrocytes. Among the cell nuclei labeled with DAPI are some small pyknotic nuclei of dead cells (arrows in C; see also Supplementary Fig. 1) which are distinguishable from the larger nuclei of live cells and were therefore excluded from the total cell counts. D and E show absence of labeling of cells, whose nuclei can be seen in (F), when the primary antibodies were omitted. Scale bar = 100 μm.

Dissociated hippocampal cells after 3 days in culture contain both βIII tubulin-positive neurons and GFAP-positive astrocytes. Among the cell nuclei labeled with DAPI are some small pyknotic nuclei of dead cells (arrows in C; see also Supplementary Fig. 1) which are distinguishable from the larger nuclei of live cells and were therefore excluded from the total cell counts. D and E show absence of labeling of cells, whose nuclei can be seen in (F), when the primary antibodies were omitted. Scale bar = 100 μm.