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. 2014 May;101(100):5–15. doi: 10.1016/j.phytochem.2014.02.010

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© 2014 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).

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Fig. 1 — Chromatographic runs (FPLC). (A) Cation exchange chromatography on SP-Sepharose. (B) Cation exchange chromatography on MonoS. (C) Cation exchange chromatography on MonoS jrPPO1(Asp¹⁰¹ → Pro⁴⁴⁴). (D) Cation exchange chromatography on MonoS jrPPO1(Asp¹⁰¹ → Arg⁴⁴⁵). Legend: , UV absorbance at 280 nm [mAU]; , UV absorbance at 410 nm [mAU]; , monophenolase activity [U/ml]; , gradient [% buffer B]; , conductivity [mS/cm] (f = ∼1.5).

Inline graphic — Chromatographic runs (FPLC). (A) Cation exchange chromatography on SP-Sepharose. (B) Cation exchange chromatography on MonoS. (C) Cation exchange chromatography on MonoS jrPPO1(Asp¹⁰¹ → Pro⁴⁴⁴). (D) Cation exchange chromatography on MonoS jrPPO1(Asp¹⁰¹ → Arg⁴⁴⁵). Legend: , UV absorbance at 280 nm [mAU]; , UV absorbance at 410 nm [mAU]; , monophenolase activity [U/ml]; , gradient [% buffer B]; , conductivity [mS/cm] (f = ∼1.5).