Fig. 2.

Analytic one- and two-dimensional SDS–PAGEs. (A) Purified jrPPO1(Asp¹⁰¹ → Pro⁴⁴⁴) and jrPPO1(Asp¹⁰¹ → Arg⁴⁴⁵). Staining: Coomassie Brilliant Blue. M_w marker [kDa] in the middle lane. (B) In-gel activity staining by l-tyrosine. M_w marker [kDa] in the middle lane. (C) Coomassie-stained two-dimensional polyacrylamide gel of jrPPO1(Asp¹⁰¹ → Pro⁴⁴⁴) and jrPPO1(Asp¹⁰¹ → Arg⁴⁴⁵), which were separated in the first dimension on an immobilized pH gradient strip (pH 4.0–7.0) and in the second dimension on a 12% polyacrylamide gel.