Table 1.

List of peptides found by nanoUHPLC–ESI-MS/MS protein identification experiments of jrPPO1(Asp¹⁰¹ → Pro⁴⁴⁴). Sequence coverage: 96%.

Start–End	Sequence	Modifications	XCorr	Delta Mass [ppm]	Enzyme for digestion	Peptide mass
101–108	DPVSAPEL		1.5	−0.93	Chymotrypsin	826.406
101–110	DPVSAPELTL		1.97	0.46	Chymotrypsin	1040.539
101–131	DPVSAPELTLcSEADLPAGALPVNccPPTSK	C11(Carbamidomethyl); C25(Carbamidomethyl); C26(Carbamidomethyl)	6.51	−1.72	Trypsin	3265.524
111–136	cSEADLPAGALPVNccPPTSKKIKDF	C1(Carbamidomethyl); C15(Carbamidomethyl); C16(Carbamidomethyl)	5.5	−1.18	Chymotrypsin	2874.368
122–136	PVNccPPTSKKIKDF	C4(Carbamidomethyl); C5(Carbamidomethyl)	3.4	0.91	Chymotrypsin	1789.887
132–146	KIKDFVLPSQNTPLR		4.86	−2	Trypsin	1755.000
133–146	IKDFVLPSQNTPLR		5.05	−2.46	Trypsin	1626.905
135–146	DFVLPSQNTPLR		3.06	−118	Trypsin	1385728
139–153	PSQNTPLRVRPAAHL		1.62	2.05	Chymotrypsin	1655.925
146–153	RVRPAAHL		2.03	−1.21	Chymotrypsin	918.550
146–158	RVRPAAHLVDNDY		3	1.99	Chymotrypsin	1524.782
146–162	RVRPAAHLVDNDYIAKY		4.22	0	Chymotrypsin	2000.059
147–161	VRPAAHLVDNDYIAK		5.17	−1.54	Trypsin	1680.892
154–162	VDNDYIAKY		2.51	0.48	Chymotrypsin	1099.519
162–170	YNKGIELmK	M8(Oxidation)	2.17	−1.16	Trypsin	1110.573
163–168	NKGIEL		1.85	−0.4	Chymotrypsin	672.380
163–180	NKGIELmKSLPADDPRSF	M7(Oxidation)	5.07	−1.8	Chymotrypsin	2033.021
163–180	NKGIELMKSLPADDPRSF		4.73	−0.13	Chymotrypsin	2017.030
169–180	mKSLPADDPRSF	M1(Oxidation)	3.53	−0.13	Chymotrypsin	1378.654
169–180	MKSLPADDPRSF		2.91	−1.08	Chymotrypsin	1362.658
171–178	SLPADDPR		1.7	−1.54	Trypsin	869.422
173–180	PADDPRSF		1.65	−0.44	Chymotrypsin	903.408
181–190	TQQANVHcAY	C8(Carbamidomethyl)	2.21	−1.43	Chymotrypsin	1190.512
181–195	TQQANVHcAYcDGAY	C8(Carbamidomethyl); C11(Carbamidomethyl)	2.5	−1.49	Chymotrypsin	1756.690
196–203	TQVGFPDL		2.03	0.15	Chymotrypsin	875.439
196–205	TQVGFPDLSL		2.18	0.34	Chymotrypsin	1075.555
219–226	YYVYFFEK		2.61	−2.57	Trypsin	1157.540
219–230	YYVYFFEKILGK		2.12	−1.23	Trypsin	1568.825
224–231	FEKILGKL		2.68	0.42	Chymotrypsin	946.585
225–237	EKILGKLIGDPTF		3.38	−0.64	Chymotrypsin	1429.817
229–237	GKLIGDPTF		2.45	−0.14	Chymotrypsin	946.512
231–268	LIGDPTFALPFWNWDSPPGmQLPSLYAVSNSAIYDPLR	M20(Oxidation)	6.57	0.27	Trypsin	4264.099
231–268	LIGDPTFALPFWNWDSPPGMQLPSLYAVSNSAIYDPLR		4.55	−1.96	Trypsin	4248.094
245–256	DSPPGmQLPSLY	M6(Oxidation)	2.26	1.09	Chymotrypsin	1319.608
245–256	DSPPGMQLPSLY		2.72	0.57	Chymotrypsin	1303.612
257–267	AVSNSAIYDPL		1.69	−0.12	Chymotrypsin	1148.571
257–280	AVSNSAIYDPLRNANHQPPTIIDL		2.94	1.12	Chymotrypsin	2618.348
265–280	DPLRNANHQPPTIIDL		3.09	−1.55	Chymotrypsin	1812.945
268–280	RNANHQPPTIIDL		2.32	0.19	Chymotrypsin	1487.784
268–299	RNANHQPPTIIDLDYGETSESTTTTDQVPSNL		7.47	−3.7	Chymotrypsin	3513.636
269–300	NANHQPPTIIDLDYGETSESTTTTDQVPSNLK		7.62	−0.96	Trypsin	3485.640
281–299	DYGETSESTTTTDQVPSNL		2.95	2.68	Chymotrypsin	2043.881
300–316	KImYRQmVSGAKNPTLF	M3(Oxidation); M7(Oxidation)	1.47	0.1	Chymotrypsin	2015.033
301–311	ImYRQmVSGAK	M2(Oxidation); M6(Oxidation)	2.21	−0.22	Trypsin	1314.642
304–316	RQmVSGAKNPTLF	M3(Oxidation)	4.59	1.53	Chymotrypsin	1463.757
304–316	RQMVSGAKNPTLF		4.15	−0.15	Chymotrypsin	1447.760
304–317	RQmVSGAKNPTLFF	M3(Oxidation)	3.57	0.8	Chymotrypsin	1610.825
304–317	RQMVSGAKNPTLFF		3.47	0.88	Chymotrypsin	1594.830
312–322	NPTLFFGSPYR		3.89	−2.13	Trypsin	1297.642
322–344	RAGDEPDPGAGTIESTPHNNIHL		6.32	−0.98	Chymotrypsin	2397.128
322–345	RAGDEPDPGAGTIESTPHNNIHLW		8.24	−0.92	Chymotrypsin	2583.207
345–361	WTGDDTQPNIENmGNFY	M13(Oxidation)	1.9	2.95	Chymotrypsin	2016.821
346–360	TGDDTQPNIENmGNF	M12(Oxidation)	3.18	0.09	Chymotrypsin	1667.673
346–360	TGDDTQPNIENMGNF		2.79	0.34	Chymotrypsin	1651.679
346–361	TGDDTQPNIENmGNFY	M12(Oxidation)	3.34	−0.1	Chymotrypsin	1830.736
346–361	TGDDTQPNIENMGNFY		3.3	2.27	Chymotrypsin	1814.745
361–370	YSAGRDPIFF		2.84	1.15	Chymotrypsin	1171.567
362–370	SAGRDPIFF		2.25	0.63	Chymotrypsin	1008.503
362–380	SAGRDPIFFAHHSNVDRmW	M18(Oxidation)	4.74	0.12	Chymotrypsin	2258.044
366–378	DPIFFAHHSNVDR		4.07	−2.16	Trypsin	1553.734
366–389	DPIFFAHHSNVDRmWTIWKTLGGK	M14(Oxidation)	3.01	−1.14	Trypsin	2871.424
371–380	AHHSNVDRmW	M9(Oxidation)	2.93	−1.01	Chymotrypsin	1267.550
371–380	AHHSNVDRMW		3.42	−0.36	Chymotrypsin	1251.556
371–383	AHHSNVDRmWTIW	M9(Oxidation)	4	−1.68	Chymotrypsin	1667.759
371–383	AHHSNVDRMWTIW		2.33	0.11	Chymotrypsin	1651.767
371–386	AHHSNVDRmWTIWKTL	M9(Oxidation)	2.48	−1.23	Chymotrypsin	2009.986
379–384	mWTIWK	M1(Oxidation)	1.88	−2.53	Trypsin	879.429
379–384	MWTIWK		1.88	−2.18	Trypsin	863.434
379–389	mWTIWKTLGGK	M1(Oxidation)	2.36	−1.39	Trypsin	1335.699
384–399	KTLGGKRKDITDPDWL		3.77	−1.36	Chymotrypsin	1841.997
387–399	GGKRKDITDPDWL		3.74	−0.94	Chymotrypsin	1499.771
387–403	GGKRKDITDPDWLNSSF		3.6	−0.87	Chymotrypsin	1934.946
390–414	RKDITDPDWLNSSFFFYDENADPVR		4.9	−0.72	Trypsin	3046.407
391–414	KDITDPDWLNSSFFFYDENADPVR		7.02	−1.3	Trypsin	2890.304
392–414	DITDPDWLNSSFFFYDENADPVR		6.48	−1.21	Trypsin	2762.210
392–416	DITDPDWLNSSFFFYDENADPVRVK		2.67	−0.2	Trypsin	2989.376
405–426	FYDENADPVRVKVKDcVDNTKL	C16(Carbamidomethyl)	6.06	2.94	Chymotrypsin	2624.298
406–426	YDENADPVRVKVKDcVDNTKL	C15(Carbamidomethyl)	7.26	−2.45	Chymotrypsin	2477.216
406–428	YDENADPVRVKVKDcVDNTKLRY	C15(Carbamidomethyl)	7.6	−1.21	Chymotrypsin	2796.383
407–426	DENADPVRVKVKDcVDNTKL	C14(Carbamidomethyl)	6.25	−1.18	Chymotrypsin	2314.156
407–428	DENADPVRVKVKDcVDNTKLRY	C14(Carbamidomethyl)	6.73	−0.72	Chymotrypsin	2633.321
415–425	VKVKDcVDNTK	C6(Carbamidomethyl)	1.48	−0.38	Trypsin	1304.675
417–427	VKDcVDNTKLR	C4(Carbamidomethyl)	3.09	0.1	Trypsin	1346.697
427–437	RYVYQDVEIPW		2.86	−0.83	Chymotrypsin	1466.718
428–439	YVYQDVEIPWLK		4.01	−0.47	Trypsin	1551.796
428–444	YVYQDVEIPWLKTKPTP		3.39	−0.83	Trypsin	2076.091
429–437	VYQDVEIPW		1.91	0.01	Chymotrypsin	1147.554
429–438	VYQDVEIPWL		1.72	−0.64	Chymotrypsin	1260.638
431–437	QDVEIPW		1.95	−0.85	Chymotrypsin	885.422
431–444	QDVEIPWLKTKPTP		3.47	−0.39	Chymotrypsin	1650.897
438–444	LKTKPTP		1.84	−0.35	Chymotrypsin	783.485