Table 3.

Comparison of methods for isolation and derivation of blood-derived and gonadal-derived PGCs.

Cells	Feeder layer	Sera/growth factors/cytokines	References
cPGCs	Irradiated BRL	FBS, CS/bFGF, SCF/secreted LIF from BRL	van de Lavoir et al. (2006a)
		FBS, CS/–/–	Yamamoto et al. (2007)
		FBS, CS/bFGF, SCF, hLIF	Choi et al. (2010)
	Irradiated STO	FBS, CS/FGF, SCF/secreted LIF from BRL	Macdonald et al. (2010)
gPGCs	CEF	FBS, CS/bFGF, SCF, IGF-I/mLIF, IL-11	Park and Han (2000)
	GSC	FBS, CS/bFGF, SCF/mLIF	Suraeva et al. (2008)
	CEF	FBS, CS/bFGF, SCF, IGF-I/mLIF, IL-11	Shiue et al. (2009)
	Inactivated MEF	FBS, CS/bFGF, SCF, IGF-I/mLIF	J. Wang et al. (2009)
	CEF	FBS/bFGF/mLIF	Wu et al. (2010)

Abbreviations: BRL = buffalo rat liver cells, STO = Sandoz inbred mouse-derived thioguanine-resistant and ouabain-resistant fibroblast, CEF = chicken embryonic fibroblasts, GSC = gonadal stromal cells, MEF = mouse embryonic fibroblasts, FBS = Fetal bovine serum, CS = chicken serum, bFGF = basic fibroblast growth factor, SCF = stem cell factor, IGF-I = insulin growth factor type I, mLIF = murine leukaemia inhibitory factor, IL-11 = interleukin-11.