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. Author manuscript; available in PMC: 2014 Apr 16.
Published in final edited form as: Development. 2008 Oct 2;135(21):3491–3499. doi: 10.1242/dev.024349

Fig. 3. Hb is necessary and sufficient to specify the first temporal identity.

Fig. 3

(A) Drosophila hb mutants lack the RP1 and RP4 neurons, but have normal RP3 and RP5 neurons (Table 1). Wild type has RP1, RP4, RP3 and RP5 neurons (see Fig. 2). (B) hb misexpression (insc-gal4 UAS-hb) generates ectopic RP1/4 neurons based on molecular markers (Table 1). (C) (Top row) Wild-type RP motoneurons express the pan-motoneuron markers phosphorylated (p) Mad (PMAD) and Late Bloomer. (Bottom row) hb misexpression (insc-gal4 UAS-hb) generates ectopic RP1/4 neurons that are double positive for pMad and Late Bloomer (100%, n=48). For all panels, a single representative hemisegment of a stage-16 CNS is shown as a maximum intensity projection. Midline, left; anterior, up. A phenotype summary is shown in the right-hand panels, with Late Bloomer expression indicated by dashed circles. Scale bars: 3 μm.