Table 3.
Characteristics of the 28 polymorphic SSR markers validated in 28 Pyrus accessions
| Locus | Repeat motif | Primer sequence (5'–3') | Size range (bp) | A | N g | N a | H o | H e | PIC |
| XY121 | (CT)8 | F: <FAM><Tail>ACTGCTTGTAACACCCAACG; R: CGGAGTCAGTTGGATATCGG | 102–158 | 0.3333 | 25 | 16 | 0.6071 | 0.8714 | 0.8460 |
| TXY86 | (AG)8 | F: <FAM><Tail>TTGGGTCTTTAAATGCCAGC; R: CCAGACGTGAGTTGTTGCC | 114–156 | 0.1667 | 28 | 17 | 0.8214 | 0.9318 | 0.9091 |
| TXY170 | (CAT)9 | F: <FAM><Tail>TGCCTTTCCATTCTCAGGTC; R: AAGCGGCTGTCTAAATGAACA | 104–144 | 0.3611 | 17 | 8 | 0.6071 | 0.7571 | 0.7095 |
| TXY88 | (AG)8 | F: <FAM><Tail>GAGGGAGAGAGGAAGCCATT; R: CACAAGCTTTGTATTCGGCA | 110–150 | 0.3472 | 18 | 11 | 0.4286 | 0.8123 | 0.7730 |
| TXY5 | (TG)8 | F: <FAM><Tail>GGAGCAATGTGTGTTGTCACT; R: CCTTGCGATCGATAATTTCC | 118–146 | 0.2222 | 22 | 9 | 0.4643 | 0.8766 | 0.8459 |
| TXY276 | (CAGCT)5 | F: <FAM><Tail>CCCTACAGAGTCATGCATCC; R: TTGATGCTGGAGACGAGAAA | 119–144 | 0.5714 | 12 | 6 | 0.4074 | 0.6408 | 0.5882 |
| TXY11 | (TC)8 | F: <FAM><Tail>CAGAATTCAACATTCACTCTCTCTC; R: GAGTAGGGATGTGTCGGCTC | 120–166 | 0.3235 | 13 | 9 | 0.2692 | 0.8228 | 0.7814 |
| TXY15 | (TC)8 | F: <HEX><Tail>CAGGCTAGGGTTCAGGGTTT; R: CCTTTGAAGCCAAAGACTCG | 146–184 | 0.2500 | 23 | 14 | 0.6429 | 0.8565 | 0.8250 |
| TXY102 | (AC)8 | F: <HEX><Tail>CTCTTAACCCTCTCTCCCCC; R: CCCGATGACTCTTGAATGGT | 159–183 | 0.2639 | 21 | 12 | 0.5000 | 0.8532 | 0.8204 |
| TXY16 | (TC)8 | F: <HEX><Tail>TTGCTGAAGCTTCTCTTCTTCTC; R: GAGCCCACAAGGGTTCAATA | 160–210 | 0.2500 | 19 | 8 | 0.1429 | 0.7766 | 0.7362 |
| TXY267 | (TC)9 | F: <HEX><Tail>GTCTTAGCTTCTGCTCCCGA; R: AACCTGATGAGAATGACGGG | 149–195 | 0.1944 | 21 | 12 | 0.4286 | 0.8909 | 0.8625 |
| TXY63 | (CT)8 | F: <HEX><Tail>ACCTGTCATCACTTCTACTGGTTA; R: AGCCTACAAACATGCATCCC | 169–189 | 0.1944 | 24 | 11 | 0.4643 | 0.8903 | 0.8623 |
| TXY94 | (AG)8 | F: <HEX><Tail>ACACGCGCACACAGAGATAC; R: CCGTCACGTCGCTTTCTT | 172–206 | 0.2083 | 26 | 14 | 0.5000 | 0.8961 | 0.8689 |
| TXY95 | (AG)8 | F: <HEX><Tail>TACGGACAAGGACACCATGA; R: CTTGACGGTGTCAAAGGGAT | 177–205 | 0.4028 | 14 | 10 | 0.5000 | 0.7721 | 0.7240 |
| TXY236 | (TCC)6 | F: <NED><Tail>TCCACTCCAAACCCAGAAAC; R: GCTTCAGGGAAGCTGAAATG | 183–189 | 0.7571 | 4 | 2 | 0.2222 | 0.3075 | 0.2562 |
| TXY185 | (CAG)7 | F: <NED><Tail>GGAGGACCAACAGCAACATT; R: AGCAAGATCAAGCCAGGTGT | 181–196 | 0.3889 | 11 | 6 | 0.4643 | 0.7682 | 0.7134 |
| TXY2 | (AC)9 | F: <NED><Tail>ACGCTTCAGGTTTGGACTTC; R: TCAACCTGGACCATACATTCA | 172–206 | 0.2083 | 22 | 10 | 0.5714 | 0.8656 | 0.8325 |
| TXY280 | (AAGG)5 | F: <NED><Tail>CGTCCAAACTCCTCAGCTTC; R: CTCTGTCTGTCTGGTCGGGT | 187–215 | 0.2778 | 17 | 8 | 0.6786 | 0.8253 | 0.7845 |
| TXY150 | (TC)9 | F: <NED><Tail>GCCTTGGGCTTCTTCTTCTT; R: TCGTCGTGTGATTCGACTTC | 190–216 | 0.4583 | 14 | 8 | 0.3929 | 0.7370 | 0.6898 |
| TXY219 | (GTG)6 | F: <NED><Tail>CTAAGAAGCCGTTCCGATGA; R: GTCTCCCATCCTCCTCTTCC | 194–218 | 0.5556 | 11 | 7 | 0.3929 | 0.6792 | 0.6304 |
| TXY45 | (GA)8 | F: <NED><Tail>CAGACCCCAATTCAACGTCT; R: TCTTCTGGACATGGAGGACC | 184–230 | 0.4167 | 14 | 7 | 0.5000 | 0.7409 | 0.6956 |
| TXY214 | (ACC)6 | F: <PET><Tail>AACCTAAACGCATTGCAACC; R: AAGATGTGAAACCGAAACCG | 202–235 | 0.2361 | 16 | 9 | 0.8214 | 0.8474 | 0.8109 |
| TXY25 | (TC)8 | F: <PET><Tail>TCGATTCGATCAGGTGCTAA; R: CCGTCTCTCTTTCTCCGTTG | 145–235 | 0.3194 | 18 | 10 | 0.3929 | 0.7773 | 0.7338 |
| TXY138 | (GCC)6 | F: <PET><Tail>AGCATTTCCGCTGTCAGAAT; R: ATGAAGTCGTCCAGAGCGTT | 218–230 | 0.3750 | 11 | 5 | 0.5357 | 0.7565 | 0.7014 |
| TXY48 | (GA)8 | F: <PET><Tail>ACTAGGCAAACCAATCCAGC; R: TTGGGATCTTGTGAAGAGGG | 221–245 | 0.4032 | 13 | 8 | 0.2500 | 0.7385 | 0.6923 |
| TXY144 | (GAG)11 | F: <PET><Tail>ACGGAGAAGAAGCAGCAGAG; R: ACAATCTCATCCTCAACGCC | 216–228 | 0.5161 | 8 | 5 | 0.1667 | 0.7048 | 0.6490 |
| TXY104 | (AC)8 | F: <PET><Tail>CCCTTGATACTCAAACCCTCC; R: GATCGTTCCTTCACCAGCAT | 238–270 | 0.3286 | 17 | 13 | 0.8889 | 0.8519 | 0.8204 |
| TXY164 | (TGC)5 | F: <PET><Tail>CACACGAATAGCTCCTGCAA; R: AGCATCAGCAACAGCAACAG | 241–268 | 0.2917 | 16 | 9 | 0.6071 | 0.8279 | 0.7903 |
| Mean | 0.3437 | 16.96 | 9.429 | 0.4882 | 0.7884 | 0.7483 |
<FAM>, <HEX>, <NED>, and <PET> represent four different fluorescent labels; <Tail> represents the M13 universal primer (TGTAAAACG ACGGCCAGT) added to the 5' end of forward primers. Shown for each primer pair are the repeat motif, primer sequence, size range (bp), major allele frequency (A), number of genotypes (N g), number of alleles (N a), observed heterozygosity (H o), expected heterozygosity (H e), and polymorphism information content (PIC)