Figure 2. SAHA treated Breg-depleted PBMC from HIV_EC and HIV_ART exhibit higher frequencies of anti-HIV CTL-competent CD8⁺ T cells.

(a) 500 nM SAHA-treated total or Breg-depleted PBMC from HIV_EC (n = 4) and HIV_ART (n = 6) were cultured for 4 days and the frequency CD107a⁺CD8⁺ T cells determined by flow cytometry. (b) After 4 days in culture, by flow-cytometry using an HLA-A*0201 MHC-I HIV Dextramer® (Immudex), the frequency of HIV_gagSL9⁺ CD8⁺ T cells was determined; left panel depicts representative dot-plots demonstrating specific binding and right panel shows the summary of results (A2^pos = HLA-A*2 positive, A2^neg = HLA-A*2 negative). p values for differences as calculated by paired one-tailed t test (Graphpad Prism software) are indicated.