Figure 3. Association between elevated frequency of CTL–competent T cells, clearance of infected CD4⁺ T cells and reduced viral DNA.

(a) 500 nm SAHA-treated total or Breg-depleted PBMC from HIV_EC (n = 4) and HIV_ART (n = 5) were cultured for 4 days and the frequency of infected CD4⁺ T cells was determined by binding to KC57-antibody. (b) In HIV_ART subjects (n = 5), relative levels of HIV DNA between SAHA-treated total or Breg-depleted PBMC were determined by qPCR with LTR hybridizing primers after 4 days in culture. p values as calculated by paired two-tailed t test (Graphpad Prism software) are indicated.