Figure 4. SAHA-treated Breg-depleted PBMC from HIV_EC and HIV_ART exhibit upregulated expression of antigen-presenting molecules and proliferation of CD4+ T cells.

After 4 days in culture, (a) the expression of MHC-II and MHC-I/II on B cells and dendritic cells (LIN⁻CD11c⁺HLA-DR⁺) respectively was determined by flow cytometry in (b) SAHA-treated total or Breg-depleted PBMC from HIV_EC, (n = 4, upper panel) and HIV_ART, (n = 4, lower panel). The gating strategy and representative histogram overlays are depicted in Figure 3a. (c) VPD450-proliferation dye labeled total or Breg-depleted PBMC were stimulated with SAHA (500 nM, Figure 1b right panel, n = 5) and after 4 days in culture proliferation of CD4⁺ T cells was determined by flow cytometry. p values for differences in CD4⁺ T cell proliferation as calculated by paired two-tailed t test (Graphpad Prism software) are indicated.