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. 2014 Apr 16;9(4):e94547. doi: 10.1371/journal.pone.0094547

Figure 7. MZC prevents HPV-16 PsV anorectal infection in mice.

Figure 7

A) Ketamine/xylazine-anesthetized Balb/C mice were treated in the anorectal area with Conceptrol or D-PBS (no Conceptrol). Mice were challenged with HPV-16 PsV to determine the optimal time for conceptrol treatment that gives the best luminescence signal. B) Ketamine/xylazine-anesthetized Balb/C mice received Conceptrol in the anorectal area and were treated with the indicated formulations (HEC placebo for HPV-16 PsV control or MZC) in the anorectal area at different time points before and after HPV-16 PsV challenge (15 animals/treatment). (A and B) In vivo luciferase expression was measured 48 h after challenge using the IVIS Lumina and is expressed as mean luminescence in photons per second per centimeter square per steridian + SD (*P<0.05 vs. HEC, Mann Whitney U test). C) CG PK in rectal washes from mice treated with MZC formulation was determined at 0.5, 2, and 8 h (n = 6 or 8 per time point) after MZC application. The graph shows CG concentration (mean μg/ml ± SD) per time point.