Table 3. Kinetic parameters of AcCR for reduction of 4′-chloroacetophenone and oxidation of isopropanol.
| Substrates | Km c (mM) | Vmax (mM/min) |
| NADH | 0.66±0.021 | 0.21±0.008 |
| 4′-Chloroacetophenonea | 4.13±0.157c | -- |
| NADPH | 0.026±0.001 | 0.17±0.005 |
| 4′-Chloroacetophenoneb | 2.96±0.087 | -- |
| NAD+ | 1.33±0.045 | 0.069±0.003 |
| Isopropanola | 48.32±0.986 | -- |
| NADP+ | 1.12±0.035 | 0.074±0.004 |
| Isopropanolb | 67.82±2.321 | -- |
Kinetic constants were spectrophotometrically analyzed by determining the initial reaction rate at 340 nm at different substrate concentrations. Reactions were carried out in the optimal conditions. The dependence of AcCR's activity on substrate concentrations and the corresponding Michaelis–Menten curve or Hill curve were illustrated in Figures S2–5.
a
Determination for the NAD(H)-linked reaction.
b
Determination for the NADP(H)-linked reaction.
c
The apparent Km value was considered as S0.5.