Skip to main content
. 2014 Apr 16;9(4):e94091. doi: 10.1371/journal.pone.0094091

Figure 4. Bmsage protein interacts with SGF1 by far-western blot and ELISA analysis.

Figure 4

A. Interaction analysis of Bmsage with SGF1. (a1) Purified Bmsage-GST as prey protein was incubated with PBS (as a control) and detected by anti-GST antibody. (a2) purified SGF1 as prey protein was incubated with purified Bmsage-GST (bait protein) and detected by anti-GST antibody. The purified GST protein was used as a positive control. BSA protein was used as a negative control. B. The binding of Bmsage and SGF1 was determined by ELISA. The BSA protein was used as a negative control. The results are expressed as the means ± SD of three independent experiments. Asterisk indicate that the value is significantly different from control (** p<0.01).