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. 2014 Apr 16;9(4):e94035. doi: 10.1371/journal.pone.0094035

Figure 3. Identification of GK and GKRP isoforms expressed in tanycytes.

Figure 3

A, GK sequences were amplified by RT-PCR using primers that were specific for pancreatic GK (856 bp; top panel), liver GK (388 bp; middle panel), and β-actin (353 bp; bottom panel). PCR products amplified from liver (lane 1), pancreas (lane 2), hypothalamus (lane 3), tanycytes in culture (lane 4) cDNA. Negative controls consisted of RT(-) of hypothalamus (lane 5) and tanycytes (lane 6). B, Immunoblots of GK in total protein extract from pancreas (lane 1), hypothalamus (lane 2) and tanycyte cultures (lane 3), negative control using pre-absorbed antibody (lane 4). C, RT-PCR to amplify GKRP (418 pb; upper-panel) and β-actin (353 bp; lower-panel) using liver (lane 1), hypothalamus (lane 2), tanycytes (lane 3) and pancreas (lane 4) cDNA. Negative controls consisted of RT(-) of hypothalamus (lane 5) and tanycytes (lane 6) cDNA. D, Immunoblots of GKRP in total protein extract from liver (lane 1), hypothalamus (lane 2) and tanycyte culture (lane 3), using the omission of anti-GKRP (lanes 4).