Figure 2. The ability of cAMP analogs to inhibit hepatocyte apoptosis coincides with inhibition of Nod and bile acid uptake.

A: Freshly isolated hepatocytes were incubated with vehicle or cAMP analogs (50 µM) for 15 min before addition of 150 nM nodularin followed by co-incubation for another 60 min. The cells were next fixed and apoptosis scored as described in Figure 1. B: Freshly isolated hepatocytes were incubated with vehicle or cAMP analogs (50 µM) for 15 min before addition of [³H]-nodularin. The cells were incubated for another 5 min before separation of cells from supernatant and determination of intracellular radioactivity as described in the Experimental section. C: Freshly incubated hepatocytes were treated as in B, but added [¹⁴C]-glycocholic acid instead of nodularin. Cells were separated from supernatant by gentle filtration and intracellular radioactivity determined. The concentration of cAMP analogs in B and C is 50 µM. The data are average from 3-5 experiments and SEM.