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. 2014 Apr 16;9(4):e94926. doi: 10.1371/journal.pone.0094926

Figure 4. 8-pCPT-cAMP inhibits Nod-mediated apoptosis in OATP1B1- or 1B3 transfected HEK293T cells.

Figure 4

HEK293T cells were transfected with OATP1B1 or OATP1B3, and treated with the OATP1B1/1B3 inhibitor rifamycin SV, or the cAMP analogs 8-pCPT-cAMP or 6-MB-cAMP for 15 min before addition of Nod (0.1 µM for OATP1B1 and 0.6 µM for OATP1B3). After another 90 min incubation, the cells were fixed, and apoptosis assessed as described in the methods section. The results show cell death relative to that induced by Nod without cAMP analogs, and are mean and s.e.m. of 3-5 experiments.