Figure 1. Induction of hematoma and clot formation in the bone marrow.

(A) Induction of bleeding results in clot formation within 5 minutes as evidenced by Masson-Goldner staining. Bar represents 500 µm. The enlarged inset below shows the blood clot. Bar represents 100 µm. (B) The clot is still present, albeit partially reorganized at 24 hours after bleeding induction. Bar represents 500 µm. Enlarged inset is shown below. Bar represents 100 µm. (C) After 72 hours the clot resolved. (D) Thrombin staining of the bone shown in B confirms the presence of thrombin (in red) in the clot area. (E) Quantification of the change in hematoma size at different time points. After induction of bleeding in the bone marrow, mice were euthanized at the times mentioned, and tibiae examined. n = 3–4 mice/time point.