Figure 4. Comparison of manual and automatic filament selection options.

A. Plot of average filament length as a function of pixel threshold (“Minimum number of pixels…”) setting comparing automatic (solid circles) and manually (open circles) accepted filament reconstructions. B. Percent of filaments manually accepted as a function of the pixel threshold setting. C. Number of filaments reconstructed using the pixel gap function (solid circles) and without pixel skipping (open circles) as a function of the pixel threshold setting. D. Average filament length for the two cases in panel C showing no significant difference between them.