| Strains |
|
|
|
M. chelonae ATCC 35752 |
M. chelonae reference strain |
ATCC |
|
M. chelonae 9917 |
Glutaraldehyde-resistant isolate of M. chelonae
|
[21]
|
|
M. abscessus ATCC 19977 |
M. abscessus reference strain |
ATCC |
| Plasmids |
|
|
| pOMK |
pBluescript KS- derivative carrying a mycobacterial origin of replication and a Kan resistance gene |
[43]
|
| pOMK-zeo |
pOMK derivative carrying the zeo resistance gene from pEM7/Zeo |
This study |
| pOMK_4691 |
pOMK derivative expressing MCH_4691c under control of its own promoter |
This study |
| pOMK_[4691_4689] |
pOMK derivative expressing MCH_4691c, MCH_4690c and MCH_4689c under control of their own promoter |
This study |
| pJV53 |
Recombineering plasmid carrying the Che9c gp60 and gp61 genes under control of the acetamidase promoter |
[20]
|
| pJV53-xylE |
pJV53 derivative carrying the xylE gene |
This study |
| pEM7/Zeo |
Plasmid carrying the Zeo resistance cassette |
Invitrogen |
| pUC4K |
Plasmid carrying the Kan resistance cassette |
GE Healthcare |
| pPR27-xylE |
Mycobacterial shuttle plasmid carrying the sacB counterselectable marker, a Gen resistance cassette and xylE
|
[22]
|
| pPR27-4689-KX |
pPR27-xylE derivative carrying MCH4689c::kan
|
This study |
| pPR27-4690-KX |
pPR27-xylE derivative carrying MCH4690c::kan
|
This study |
| pPR27-4691-KX |
pPR27-xylE derivative carrying MCH4691c::kan
|
This study |
| pPR27-4689-ZX |
pPR27-xylE derivative carrying MCH4689c::zeo
|
This study |
| pPR27-4690-ZX |
pPR27-xylE derivative carrying MCH4690c::zeo
|
This study |
| pPR27-4691-ZX |
pPR27-xylE derivative carrying MCH4691c::zeo
|
This study |
