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. 2014 Apr 16;9(4):e94951. doi: 10.1371/journal.pone.0094951

Table 3. Counterselection efficiency of the Ts-sacB system in M. chelonae ATCC 35752.

Recovered colonies
Transformant 7H11-OADC 7H11-OADC 7H11-OADC 7H11-OADC Counterselection
Zeo (30°C) Zeo (37°C) Zeo/Suc (30°C) Zeo/Suc (37°C) efficiency (a)
MCH (ATCC) 9.7×108 4.1×108 1.6×107 4.9×105 3.38×10−4
pPR27-4691-ZX (+/−1.5×10−4)
(a)

The experiment was conducted on three independent transformants and mean counterselection efficiencies +/− standard deviations are indicated.