Table 3. Counterselection efficiency of the Ts-sacB system in M. chelonae ATCC 35752.
Recovered colonies | |||||
Transformant | 7H11-OADC | 7H11-OADC | 7H11-OADC | 7H11-OADC | Counterselection |
Zeo (30°C) | Zeo (37°C) | Zeo/Suc (30°C) | Zeo/Suc (37°C) | efficiency (a) | |
MCH (ATCC) | 9.7×108 | 4.1×108 | 1.6×107 | 4.9×105 | 3.38×10−4 |
pPR27-4691-ZX | (+/−1.5×10−4) |
The experiment was conducted on three independent transformants and mean counterselection efficiencies +/− standard deviations are indicated.