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. 2014 Apr 16;9(4):e95240. doi: 10.1371/journal.pone.0095240

Figure 3. Uptake of D/exo into recipient cells.

Figure 3

(A) Representative fluorescence microscopy of GFP-S exposed to PKH26-labeled D/exo for 0 min and 30 min. (B) Representative flow cytometry of GFP-S incubated with PKH26-labeled D/exo for 30 min, 60 min, 90 min, 120 min, and 150 min. Nearly 7.8%, 15.3%, 20.4%, 30.6%, and 34.3% of GFP-S showed PKH26 fluorescence (green line) with respect to the cells treated with unlabeled exosomes (black line). (C) Representative confocal microscopy of GFP-S exposed to PKH26-labeled D/exo for 24 h. (a) Green signal from GFP-S. (b) Red signal from PKH26-labeled D/exo. (c) Overlay of a and b. Arrows indicate punctiform signal, probably standing for docked D/exo. Arrowheads represent diffuse signal, likely from the internalization and diffusion of PKH26. (d) Green signal from control GFP-S with unlabeled D/exo. (e) No red signal can be detected in unlabeled D/exo. (f) Merging of d and e. Three experiments were carried out with similar results.