Table 3. Transcript abundance of selected genes as determined by Illumina sequencing and real time PCR.
Illumina Sequencing | Real Time PCR | |||||
Gene | In Vivo Matured # Reads ± SE | In Vitro Matured # Reads ± SE | P-Value | In Vivo Matured Relative Amount ± SE | In Vitro Matured Relative Amount ± SE | P-Value |
ATG4 | 7.8 | 17.0 | NS | 3.2±0.5 | 3.3±0.5 | NS |
HSP90AA1 | 4.5 | 13.9 | NS | 7.7±0.6 | 5.8±0.6 | <0.06 |
MRPS36 | 43.7 | 23.8 | NS | 3.85±0.4 | 0.7±0.4 | <0.001 |
PDHX | 99.8 | 69.1 | NS | 7.9±1.1 | 4.2±1.1 | <0.03 |
SLC7A3 | 15.7 | 43.6 | <0.02 | 1.8±0.4 | 3.6±0.5 | <0.02 |
SOX4 | 62.2 | 21.6 | <0.06 | 4.7±0.2 | 4.2±0.2 | NS |
UBAP2 | 26.5 | 86.0 | NS | 5.3±0.5 | 3.8±0.6 | <0.09 |
ZP2 | 141.2 | 1,134.2 | <0.06 | 84.1±16.8 | 272.0±16.8 | <0.001 |