Table 2. Primers used in molecular cloning of Bactrocera dorsalis' ryanodine receptor cDNA and diagnostic PCR.

Primer name	Primer sequence (5'-3')	Description (cDNA position)
P1-F	GCAAGAACATAGCCAAGGTGAG	RT-PCR product P1 (600–2181)
P1-R	TCCATCTACTTGACCAACAAAAATA
P2-F	GGGGAGGAAACGGTGTAGGTGAT	RT-PCR product P2 (2318–2810)
P2-R	GAAGAAGGAAGTGCGACACCAGAAG
P3-F	TACGACATACAACTGCGGCATAGGG	RT-PCR product P3 (6238–7559)
P3-R	GGACGGCGGATAAGAAGACGAATGA
P4-F	GAAAGTGGATGGTCCTATGGCGAGA	RT-PCR product P4 (8815–10 076)
P4-R	TGTGTGAGCGTTCCATCAGTTCCTA
P5-F	TTGTTCGGGACATTTATTCG	RT-PCR product P5 (10 739–11 365)
P5-R	CTTGCGTCTTGTCCTTGCCTTTATC
P6-F	TGGGAATAGCAATACTCCGTGGTGG	RT-PCR product P6 (12 011–12 594)
P6-R	GAAACCCCCAACAGCGTCCCATAAA
P7-F	GTTTAGCCCGATTTTTAGAGACAGC	RT-PCR product P7 (13 094–14 169)
P7-R	GAGGAAACTAACGGCACGAT
RyR-5'-R1	TTTCTCGCCTTCAGACCTTTGTTTACT	5'RACE product 5'-End (1–675)
RyR-5'-R2	ATAAAGCAATGTCCTGTGACCAGAACC
RyR-3'-F1	TAAAGGAGTGCGGGCAGGAGGAGGAA	3'RACE product 3'-End (15 159–15 753)
RyR-3'-F2	CCCGACGGAGATGACTACGAAGTTTAT
PCRI-F	TGAATAGCAATTTTGACCGAGCACC	overlapping cDNA fragment PCRI (177–5458)
PCRI-R	TGCAGCGATCTTCTTCGGGTATGTG
PCRII-F	CTCTTTCTGCTGCTGTTCTCCCTAC	overlapping cDNA fragment PCRII (5237–10 073)
PCRII-R	GTGAGCGTTCCATCAGTTCCTA
PCRIII-F	TTCGTAATCGTCTTAGTGCCTTTG	overlapping cDNA fragment PCRIII (9677–15 626)
PCRIII-R	TAACTACCTCCACCACCTCCTGATA
ASIa-F	CAGGTGAAGGCAATGAGGGA	diagnostic PCR for exon a
ASIa-R	ATCTAAGAACACGCCGACAAT
ASIb-F	CAGGTGAAGGCAATGAGG	diagnostic PCR for exon b
ASIb-R	ACCTTCCTCATACCGAACACC
ASII-F1	CATCTAGTTTACCAAGTGTTTCC	diagnostic PCR for the presence of exon c
ASII-R1	ACATCCGTAAGAACGCCAAC
ASII-F2	TACGCAATAAGGTCCGAATA	diagnostic PCR for the absence of exon c
ASII-R2	GTTGGGTATTGGTTCTGTAA
ASIII-F1	GCCAATCCAAACCACAAATCAACGA	diagnostic PCR for the presence of exon d
ASIII-R1	ACTCCTCCTTTAGATGGCTTGTGTA
ASIII-F2	TCCAAACCACAAATCAGTGAAAGCA	diagnostic PCR for the absence of exon d
ASIII-R2	AGTCATAAGGAACAAGTTGAGGAT
ASIV-F1	TACATTCCAAGTGCGGGTGC	diagnostic PCR for the presence of exon e
ASIV-R1	AGCGTCCCATAAACGAGAAT
ASIV-F2	TAGCAATACTCCGTGGTGGT	diagnostic PCR for the absence of exon e
ASIV-R2	GAACCTACACCAAGACCTTCTGCCT