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. 2014 Apr 16;9(4):e95222. doi: 10.1371/journal.pone.0095222

Figure 2. UV-visible and Resonance Raman spectra of the Fe-S cluster formed in E. coli IscU via RIC.

Figure 2

A. Visible spectra of IscU (50 µM) depicting the Fe-S cluster formed upon anaerobic incubation with RIC (150 µM), IscS (4 µM) and L-cysteine (3 mM). The spectra were collected every 15 min for a period of 105 min (bottom to top). B. Resonance Raman spectra of a reaction mixture containing IscU (50 µM), L-cysteine (3 mM), IscS (4 µM), DTT (4 mM) and RIC (150 µM) (a), and of a reaction mixture with all components, except RIC (b). Spectra were acquired at −190°C with 457 nm excitation, a laser power of 13 mW and accumulation time of 40 s.