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. 2014 Apr 16;9(4):e95222. doi: 10.1371/journal.pone.0095222

Figure 3. Demetalation of RIC iron with iron chelators.

Figure 3

Independent samples of the as-isolated E. coli RIC (25 µM) (A,B) and of fully reduced RIC (C) were mixed with increasing concentrations of desferrioxamine (A) and 2,2′ dipyridyl (B and C) and incubated overnight (∼18 h). The absorbance of the bands at 420 and 522 nm were used to calculate the percentage of ferric and ferrous iron atoms released from RIC as described in Material and Methods. Results are mean of three independent assays with error bars representing standard errors.