Table 1. Sequences of HBV-specific primers, tags and adaptors used for ultra-deep pyrosequencing.

	Position from EcoRI site	HBV specific target sequence	Tag sequences¶	Adaptor sequences€
PCR 1	1606 (+) [1606–1625]	5′-GCATGGAGACCACCGTGAAC-3′	No tag	No adaptor
	1974 (−) [1974–1955]	5′GGAAAGAAGTCAGAAGGCAA-3′	No tag	No adaptor
PCR 2	1653 (+) [1653–1672]	5′-CGTATCGCCTCCCTCGCGCCATCAG-3′	ACACGACGACT1	CAT AAG AGG ACT CTT GGA CT
			ACACGTAGTAT2
			ACACTACTCGT3
			ACGACACGTAT4
	1959 (−) [1959–1940]	5′-CTATGCGCCTTGCCAGCCCGCTCAG-3′	No tag	GGC AAA AAC GAG AGT AAC TC

^¶Short specific sequences used to label the different samples: ^1: sample #1; ^2: sample #2; ^3: sample #3; ^4: sample #4.

^€Short specific sequence used to ligated onto the ends of the fragments. These adaptors provide priming sequences for both amplification and sequencing of the sample-library fragments.