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. 2014 Apr 16;9(4):e95181. doi: 10.1371/journal.pone.0095181

Table 1. Primers used for PCR amplification of resistance genes.

Gene Primer Sequence(5′→3′) Annealing Temp(°C) Fragment(bp) Reference
bla TEM TEM-F TCAACATTTCCGTGTCG 56 860 [27]
TEM-R CTGACAGTTACCAATGCTTA
bla SHV SHV-F ATGCGTTATATTCGCCTGTG 56 896 [27]
SHV-R AGATAAATCACCACAATGCGC
bla CTX-M-1 M-1F CCGTTTCCGCTATTACAAACCGTTG 56 944 [37]
M-1R GGCCCATGGTTAAAAAATCACTGC
bla CTX-M-2 M-2F ATGATGACTCACAGCATTCG 56 833 [38]
M-2R TCCCGACGGCTTTCCGCGTT
bla CTX-M-8 M-8F TTTGCCCGTGCGATTGG 50 368 [28]
M-8R CGACTTTCTGCCTTCTGCTCT
bla CTX-M-9 M-9F ATGGTGACAAAGAGAGTGCA 50 870 [39]
M-9R CCCTTCGGCGATGATTCTC
bla CTX-M-10 M-10F GCAGCACCAGTA AAGTGATGG 56 524 [36]
M-10R GCGATATCGTTGGTGGTACC
bla CTX-M-14 M-14F GAGAGTGCAACGGATGATG 56 941 [29]
M-14R TGCGGCTGGGTAAAATAG
bla CMY-G1 G1-F GCTGACAGCCTCTTTCTCCAC 56 1082 [40]
G1-R CCTCGACACGGRCAGGGTTA
bla CMY-G2 G2-F GGTCTGGCCCATGCAGGTGA 56 963 [40]
G2-R GGTCGAGCCGGTCTTGTTGA
bla DHA DHA-F AACTTTCACAGGTGTGCTGGGT 60 405 [30]
DHA-R CCGTACGCATACTGGCTTTGC
bla ACT ACT-F ATTCGTATGCTGGATCTCGCCACC 50 396 [31]
ACT-R CATGACCCAGTTCGCCATATCCTG
bla FOX FOX-F CACCACGAGAATAACC 50 1184 [31]
FOX-R GCCTTGAACTCGACCG
bla KPC KPC-F TGTCACTGTATCGCCGTCTAG 50 880 [26]
KPC-R TTACTGCCCGTTGACGCCCAATCC
bla GES GES-F ATGCGCTTCATTCACGCAC 56 591 [41]
GES-R CTATTTGTCCGTGCTCAGG
bla IMI IMI-F ATGTCATTAGGTGATATGGC 50 879 [32]
IMI-R GCATAATCATTTGCCGTACC
bla IMP IMP-F GGAATAGAGTGGCTTAATTCTC 50 624 [32]
IMP-R CCAAACCACTACGTTATC
bla VIM VIM-F GATGGTGTTTGGTCGCATA 50 390 [33]
VIM-R CGAATGCGCAGCACCAG
bla GIM GIM-F TCGACACACCTTGGTCTGAA 56 477 [42]
GIM-R AACTTCCAACTTTGCCATGC
bla SPM SPM-F AAATCTGGGTACGCAAACG 56 270 [22]
SPM-R AGATTATCGGCTGGAACAGG
bla SIM SIM-F TACAAGGGATTCGGCATCG 56 570 [42]
SIM-R TAATGGCCTGTTCCCATGTG
bla NDM-1 NDM-F TGCCCAATATTATGCACCCGG 60 621 [25]
NDM-R CGAAACCCGGCATGTCGAGA
bla OXA-48 48-F TTGGTGGCATCGATTATCGG 56 743 [34]
48-R GAGCACTTCTTTTGTGATGGC