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. 2014 Apr 16;9(4):e95399. doi: 10.1371/journal.pone.0095399

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© 2014 Wei et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The mice were on chow diet or high fat diet (HFD) for 6 months, and the samples were collected in 4 h fasting state. A. Enzyme activity of IDE. HFD increased enzyme activity of IDE that was determined via the InnoZymeTM Insulysin/IDE Immunocapture Activity Assay Kit (n = 10). B. IDE protein. HFD increased IDE protein level in Western blot. The experiment was performed three times with consistent results, and a representative blot is shown. C. IDE mRNA in liver (n = 4). *P<0.05 compared with the control.