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. 2014 Apr 16;9(4):e95399. doi: 10.1371/journal.pone.0095399

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© 2014 Wei et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. IDE enzyme activity. Liver tissue was collected from DIO C57BL/6J mice were treated with pioglitazone (10 mg/kg/d) for last 2 months in 6 month HFD feeding. The samples were collected in fasting state. The IDE enzyme activity was determined (n = 10). B. IDE protein. The protein level was determined in Western blot. The experiment was performed three times with consistent results, and a representative blot is shown. C. IDE mRNA. mRNA level was determined in qRT-PCR (n = 5). D. Fasting blood insulin. E. Fasting blood glucose. F. HOMA-IR index (n = 10). *P<0.05 compared with the control.