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A. IDE protein. 1c1c7 cells were treated by palmitic acid (300 µM) for different times. In the control, cells were treated with BSA at the equal concentration for 8 hours. IDE protein was determined in whole cell lysate in Western blot. The experiment was performed three times with consistent results, and a representative blot is shown. B. IDE mRNA. IDE mRNA was in qRT-PCR (n = 3). *P<0.05 compared with the control.
