Table 1. List of primers used for quantification of the genes of interest and reference genes.

Gene	Accession number		5′-primer-3′	Tm (°C)	Size (bp)	Efficiency (%)
PCK2	NM_205470	F	GGCCGAGCACATGCTGATTT	61.7	62	102.7
		R	CCGCCATGTAACGCTTCTCA	60.7
PCK1	NM_205471	F	GGAAGTAGCCAGCTGGGTCGC	59.5	91	105.1
		R	TTGTGCGTCCTTGCATGCAGC	59.1
FBP1	NM_001278048	F	TGCTGCGGTCACCGAGTATCTCA	59.8	56	95.6
		R	GCGAACTGCCGTCCTCAGGG	59.8
HIBADH	NM_001006362	F	CCTGGGTGCTCAGGTAACAG	60.0	84	99.4
		R	TTGGGACTTGAAGGCAGCAT	59.9
ACTB	NM_205518	F	TCGCCCCAGACATCAGGGTGTGA	61.5	75	103.3
		R	TTGCTCTGGGCTTCATCACCAACGT	60.9
PPID	XM_426283	F	GTCGCACCCGTCCCCTGTAGA	60.0	93	101.8
		R	ATTCGTCCAACTCGCTCTCCCC	58.4

For every gene the NCBI accession number, the sequences and the theoretical melting temperature (Tm, °C) of the forward (F) and reverse primer (R), the size of the amplified fragment (bp) and the efficiency of amplification (%) are provided.