Table 4. Identification with LC-MS/MS of the proteins present in the 4 upregulated and 4 downregulated spots in the liver of albumen-deprived chicks at hatch compared to either the control chicks or the sham chicks or both (n = 6).
| Gene | Full name | Spot | Uniprot ID | pI | Mass(kDa) | Mascot Score | Queries | Coverage(%) | Location | control | sham | albumen-deprived | P-value |
| UP | |||||||||||||
| HSPA9 | Stress-70 protein, mitochondrial precursor | 407 | Q5ZM98 | 6.09 | 73,2 | 188 | 5 | 10 | Mt | 1.00±0.09b | 1.10±0.07ab | 1.44±0.12a | 0.010 |
| ALB | Serum albumin | P19121 | 5.51 | 69,9 | 145 | 16 | 26 | Pl | |||||
| HSPA2 | Heat shock 70 kDa protein | P08106 | 5.52 | 69,8 | 137 | 4 | 9 | Cs | |||||
| ALB | Serum albumin | 439 | P19121 | 5.51 | 69,9 | 88 | 12 | Pl | 1.00±0.11b | 1.04±0.07ab | 1.38±0.14a | 0.035 | |
| PCK2 | Phosphoenolpyruvate carboxykinase2 | 232 | P21642 | 7.56 | 71,1 | 364 | 10 | 16 | Mt | 1.00±0.05b | 1.12±0.04ab | 1.28±0.09a | 0.021 |
| LMNA | Lamin-A | P13648 | 6.50 | 73,2 | 113 | 2 | 3 | Mt, Nu | |||||
| ENO1 | Alpha-enolase§ | P51913 | 6.17 | 47,3 | 112 | 3 | 10 | Cp | |||||
| TUFM | Elongation factor Tu | P84172 | 8.98 | 38,3 | 52 | 1 | 3 | Mt | |||||
| MDH1 | Malate dehydrogenase 1 | Q5ZME2 | 6.92 | 36,5 | 36 | 1 | 3 | Cp | |||||
| ACO2 | Aconitate hydratase§ | 274 | Q8AYI3 | 8.04 | 85,8 | 261 | 6 | 9 | Mt | 1.00±0.03b | 1.01±0.02b | 1.12±0.04a | 0.010 |
| DLD | Dihydrolipoyl dehydrogenase§ | Q5ZM32 | 8.19 | 54.0 | 138 | 6 | 14 | Mt | |||||
| GLUD1 | Glutamate dehydrogenase 1 | P00368 | 8.48 | 55,7 | 65 | 4 | 12 | Mt | |||||
| ALB | Serum albumin§ | P19121 | 5.51 | 69,9 | 69 | 1 | 3 | Pl | |||||
| PCK2 | Phosphoenolpyruvate carboxykinase | P21642 | 7.56 | 71,1 | 59 | 1 | 2 | Mt | |||||
| CTH | Cystathionine gamma-lyase§ | E1BYF1 | 6.85 | 43,9 | 51 | 1 | 3 | Cp | |||||
| GLUT1 | Solute carrier family 2, facilitated glucose transporter member 1 | P46896 | 8.82 | 54,1 | 42 | 2 | 1 | Cm | |||||
| SMAD3 | Mothers against decapentaplegic homolog 3 | P84023 | 6.70 | 48,3 | 37 | 1 | 3 | Cp, Nu | |||||
| LDHB | L-lactate dehydrogenase B chain | P00337 | 7.07 | 36,3 | 36 | 1 | 3 | Cp | |||||
| DOWN | |||||||||||||
| RPLP0 | 60S acidic ribosomal protein P0§ | 1033 | P47826 | 5.71 | 34,3 | 42 | 1 | 3 | Rb | 1.00±0.07ab | 1.04±0.09a | 0.78±0.07b | 0.050 |
| FBP1 | Fructose-1,6-bisphosphatase 1§ | 1045 | Q9I8D4 | 5.22 | 18,2 | 81 | 3 | 7 | Cp | 1.00±0.10a | 0.89±0.08ab | 0.63±0.12b | 0.036 |
| HIBADH | 3-hydroxyisobutyrate dehydrogenase § | 1126 | Q5ZLI9 | 8.60 | 35,3 | 421 | 10 | 16 | Mt | 1.00±0.10a | 0.99±0.06a | 0.58±0.14b | 0.011 |
| TST | Thiosulfate sulfurtransferase | 1135 | P25324 | 6.56 | 32,3 | 92 | 7 | 15 | Mt | 1.00±0.20a | 1.16±0.16a | 0.42±0.16b | 0.007 |
The spot number (Spot), Uniprot accession number (Uniprot ID), theoretical iso-electric point (pI), theoretical molecular weight (Mass), Mascot score, number of matched peptides (Queries) and sequence coverage (Coverage) are shown. The cellular localization (Location) is also indicated: Cm = cell membrane, Cp = cytoplasm, Cs = cell surface, Mt = mitochondria, Nu = nucleus, Pl = plasma and Rb = Ribosome. The mean fluorescence (Cy3 or Cy5) of the different spots normalized according to the fluorescence signal of the pooled sample (Cy2) ± SEM are shown in the table for the control, sham and albumen-deprived chicks. a–b Within a row, treatment means with different superscript are significantly different (P<0.05). P-values of effect of treatment are added in a separate column. Most of the identified spots contained just one protein, but several enclosed multiple proteins. On the other hand, some proteins were present in more than one spot. Porcine trypsine was added for fragmentation before LC-MS/MS and this was consequently identified in the samples but this was omitted for clarity. §Homologous protein was identified in different species, but results are always displayed for the chicken (Gallus gallus).