Figure 1. Mapping tissue-specific fru^M repression to courtship latency phenotypes.

Courtship latencies of P[GawB] UAS-fru^MIR latencies depicted as stacked bars. A) White bars represent latencies in ambient light, while dark gray bars represent latencies in infrared (n = 10–44 males per genotype). Purple bars indicate the difference between dark-light latencies. Green triangles indicate position of control UAS-fru^MIR/+ line. Purple arrows indicate position of P[GawB]4-57 UAS-fru^MIR. B) Colored heat map representing the percent of fru^P1-LexA(+) neurons in a given hemisphere cluster or peripheral segment that also express Gal4. Rows are P[GawB] lines in the same respective order as panel A. Columns represent the fru^P1-LexA(+) clusters in foreleg tarsal segments (T5-T1), maxillary palp (M), labellum (P), 3^rd and 2^nd antennal segments (3, 2), and brain clusters 1–15, nomenclature from 21. C) Three lines that exhibited no or little peripheral Gal4 expression and limited central overlap with fru^P1-LexA. D) Estimated relative contributions of each fru^M(+) cluster to delays in courtship latency in infrared; values are coefficients of a linear regression derived from partial least squares analysis (see Methods). Error bars represent 95% confidence intervals estimated by resampling.