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. Author manuscript; available in PMC: 2015 Apr 17.
Published in final edited form as: FEBS Lett. 2014 Jan 16;588(8):1430–1438. doi: 10.1016/j.febslet.2014.01.003

Figure 2. Ca2+/CaM regulation of Cx43 gap junctions.

Figure 2

A. Separate fura-2 and two whole cell patch clamp experimental measurements of [Ca2+]i and normalized gap junction conductance (Gj) in Cx43-N2a cells illustrate the temporal decrease in Gj (upper panel) and increase in [Ca2+]i (lower panel) during 1 µM ionomycin perfusion (1 ml/min). B. Pretreatment with 2 µM calmidazolium prevented the Ca2+-ionomycin induced reduction in Cx43 Gj. Inclusion of inhibitory CaM binding peptides Cx43p136–158 (C) or CaMKII peptide (D) in both patch pipettes at the indicated concentrations (1–2 Kd for CaM binding) also prevented the Ca2+-ionomycin induced reduction in Cx43 Gj. Adapted from Xu et al. [51] with Author’s permission from the American Physiological Society.