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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: J Invest Dermatol. 2013 Dec 6;134(5):1246–1254. doi: 10.1038/jid.2013.523

Figure 2. Revertant iPSCs from RDEB skin.

Figure 2

(a) Persistent mRNA expression of OCT4, SOX2, NANOG, LIN28, and DNMT3b, and transient mRNA expression of c-MYC and KLF4, both consistent with fully reprogrammed mutant and revertant iPSCs. (b) Protein expression in mutant iPSCs of embryonic stem cell surface markers TRA-1-60, TRA-1-81, SSEA3, SSEA4, alkaline phosphatase (AF), and transcription factors Nanog and OCT3/4. (c) Same embryonic stem cell protein expression panel in revertant iPSCs. Nuclei are stained with DAPI (blue). Lower panels show corresponding isotype controls. Scale bar = 50 μm.