Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2015 Apr 1.

Published in final edited form as: Mol Cancer Res. 2014 Jan 24;12(4):607–621. doi: 10.1158/1541-7786.MCR-13-0469

Effect of AnxA1 on incidence, types, differentiation and proliferation of glandular structures in LSC^med grafts (A–D). A, Chart depicting the incidence and type of glandular structures (prostatic glandular or acinar) scored in LSC^med untreated control, Ac2-26 treated and rAnxA1 treated grafts. B, Representative H&E images of the types of structures detected in rAnxA1 treated grafts. Black arrow indicates simple cuboidal structure. Magnification at 400×. Bar 100 µm. C, Tissue sections were analyzed by H&E for basic histology and IHC for expression of p63, CK8, AR and Ki67. Magnification at 400×. Bar 100 µm. D, Calculated percentage of p63⁺ cells and ratio of p63⁺ to CK8⁺ cells detected in the grafts. Potential to undergo de-differentiation in response to AnxA1 was assessed by increase in p63 expression compared to CK8. E, AnxA1 treated LSC^hi/med form more and larger glandular structures than controls. rAnxA1 treated LSC^med form more and larger acinar glandular structures than rAnxA1 treated LSC^hi and Ac2-26 treated LSC^med cells. Black bars represent left y-axis data and white bars represent right y-axis data. Statistical significance is indicated by *, P < 0.05; •, P < 0.01; **, P < 0.001.

Effect of AnxA1 on incidence, types, differentiation and proliferation of glandular structures in LSC^med grafts (A–D). A, Chart depicting the incidence and type of glandular structures (prostatic glandular or acinar) scored in LSC^med untreated control, Ac2-26 treated and rAnxA1 treated grafts. B, Representative H&E images of the types of structures detected in rAnxA1 treated grafts. Black arrow indicates simple cuboidal structure. Magnification at 400×. Bar 100 µm. C, Tissue sections were analyzed by H&E for basic histology and IHC for expression of p63, CK8, AR and Ki67. Magnification at 400×. Bar 100 µm. D, Calculated percentage of p63⁺ cells and ratio of p63⁺ to CK8⁺ cells detected in the grafts. Potential to undergo de-differentiation in response to AnxA1 was assessed by increase in p63 expression compared to CK8. E, AnxA1 treated LSC^hi/med form more and larger glandular structures than controls. rAnxA1 treated LSC^med form more and larger acinar glandular structures than rAnxA1 treated LSC^hi and Ac2-26 treated LSC^med cells. Black bars represent left y-axis data and white bars represent right y-axis data. Statistical significance is indicated by *, P < 0.05; •, P < 0.01; **, P < 0.001.

HHS Vulnerability Disclosure