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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Appl Microbiol Biotechnol. 2014 Feb 21;98(9):4075–4081. doi: 10.1007/s00253-014-5552-7

Figure 4. Wzy adopts distributive mechanism for polymerization.

Figure 4

(a) The process of distributive mechanims; (b) The Wzy reaction with different exposure time (15 min vs 5 min); (c) The LPS from E. coli (Δwzy) (lane 2), from Δwzy with complementary wzy gene (Lane 1) and wzz knockout strain (Lane 3); (d) the formation of Und-PP-linked di-unit. *: contaminant band coming from synthetic Und-PP-RU; The read arrow shows the intermediates of wzy reaction; the black arrow indicated the synthetic Und-PP-RU.