Blocking P-cadherin increases anoikis and decreases EOC cell attachment
in vivo. (A,B) Female nude mice were injected
i.p. with equivalent numbers of GFP-expressing control (non-targeting) SKOV3ip
cells together with neutralizing Ab to mouse P-cadherin, control IgG or with no
Ab. Mice were sacrificed at 10 days thereafter. (A) Tumor implants
that were attached to the peritoneal cavity wall were viewed under a
fluorescence stereoscope. Bar, 1 mm. (B) Numbers of tumor implants
on the peritoneal cavity wall were counted in five random 25mm2
fields per mouse. (C) GFP-expressing control SKOV3ip cells were
pre-incubated with neutralizing Ab to human P-cadherin, control IgG or with no
Ab, and then injected i.p. into mice. Mice were sacrificed at 3 days thereafter.
Floating cells in the peritoneal cavity were collected and stained with 7AAD.
Cell death within the gated population of GFP+ tumor cells was evaluated by flow
cytometric analysis of 7AAD staining.