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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: J Trace Elem Med Biol. 2013 Dec 18;28(2):151–159. doi: 10.1016/j.jtemb.2013.12.003

Figure 5.

Figure 5

Detection of hCTR1 in immunoprecipitates prepared from HEK293T/myc-CTR1 cells using anti-hCTR1 and anti-myc antibodies. Myc-CTR1 was precipitated from PNM preparations with anti-myc or anti-hCTR1 coupled to beads. Immunoprecipitates were probed with anti-hCTR1 (upper panel) or anti-myc (lower panel). Lanes 1 and 3: CTR1 in PNM samples prior to immunoprecipitation. Lanes 3 and 4: myc-CTR1 captured by and eluted from beads coated with anti-myc antibody. Lane 5 and 6: captured by and eluted from beads coated with anti-CTR1 antibody myc-CTR1. Lane 7 and 8: proteins not completely captured by the beads coated with anti-myc antibody and present in the flow through (FT). Lane 9 and 10: proteins not completely captured by the beads coated with anti-CT1 antibody and present in the flow through (FT). *Endogenous Myc; ** IgG light chain.