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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Mol Cancer Res. 2014 Jan 27;12(4):550–559. doi: 10.1158/1541-7786.MCR-13-0458

Figure 2. TWEAK induces Mcl-1 in NSCLC cell lines in an NF-κB-dependent manner.

Figure 2

Total cell lysates were prepared from serum-reduced (A) H1975 and (B) H2073 cell lines treated with TWEAK for the indicated times and immunoblotted with the indicated antibodies: Mcl-1, Bcl-xL, and phosphorylated-p65 (Ser536). Tubulin was used as a loading control. (C) Serum-reduced H2073 cells transfected ± IkBα mutant were treated with TWEAK for 24 hours. Cells were harvested, total cell lysates were prepared and immunoblotted with the indicated antibodies to both Mcl-1 and phospho-p65. All blots were run in duplicate and tubulin was used as a loading control.