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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Mol Cancer Res. 2014 Jan 27;12(4):550–559. doi: 10.1158/1541-7786.MCR-13-0458

Figure 3. TWEAK-induced NSCLC cell survival is dependent on Mcl-1 expression.

Figure 3

H1975 (A) and H2073 (B) cells were transfected with luciferase (siCont) or siRNAs targeting Mcl-1. Total lysates were collected 72 hours post-transfection and immunoblotted for Mcl-1 and alpha-tubulin. H1975 (C and E) and H2073 (D and F) cells transfected with control or siRNA constructs targeting Mcl-1 were exposed to 1 µM cisplatin for 24 hours (C and D) or 2Gy ionizing radiation (E and F) ± pre-incubation with TWEAK (100 ng/mL). Cells were sparsely seeded into 6-well dishes and allowed to grow for 7 days prior to staining with crystal violet and colony counting. A colony was defined as containing at least 50 cells. Bars represent average of three independent wells ± standard error with the non-treated (first bar) set to 1. * represents a p value < 0.05 by ANOVA with Bonferroni posttest.